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The answer lies in The very fact that there are a number of isoforms of each and every troponin subunit which might be encoded by distinctive genes, several of that happen to be expressed selectively in cardiac muscle. Whilst TnI and TnT have distinctive cardiac and skeletal isoforms, they share a common isoform of TnC: the sluggish-twitch skeletal
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The differences in AA sequences permitted improvement of quantitative assays for cTnI/T. Most cTn assays are non-competitive enzyme-joined immunosorbent assays (ELISA) determined by the sandwich principle, utilizing the large specificity and affinity of antibodies.31 The assay is based with a capture antibody which binds to a particular epitope of
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